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Feasibility of a new method to collect exhaled breath condensate in pre‐school children
Author(s) -
Rosias Philippe P. R.,
Robroeks Charlotte M.,
Van De Kant Kim D.,
Rijkers Ger T.,
Zimmermann Luc J.,
Van Schayck Constant P.,
Heynens Jan W.,
Jöbsis Quirijn,
Dompeling Edward
Publication year - 2010
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2009.00909.x
Subject(s) - exhaled breath condensate , medicine , exhaled air , asthma , condenser (optics) , bronchoalveolar lavage , allergy , wheeze , gastroenterology , lung , immunology , toxicology , physics , light source , optics , biology
Rosias PPR, Robroeks CM, van de Kant KD, Rijkers GT, Zimmermann LJ, van Schayck CP, Heynens JW, Jöbsis Q, Dompeling E. Feasibility of a new method to collect exhaled breath condensate in pre‐school children.
Pediatr Allergy Immunol 2010: 21: e235–e244.
© 2009 John Wiley & Sons A/S Exhaled breath condensate (EBC) is a promising non‐invasive method to assess respiratory inflammation in adults and children with lung disease. Especially in pre‐school children, condensate collection is hampered by long sampling times because of open‐ended collection systems. We aimed to assess the feasibility of condensate collection in pre‐school children using a closed glass condenser with breath recirculation system, which also collects the residual non‐condensed exhaled breath, and subsequently recirculates it back into the condenser. Condensate was collected before and after breath recirculation in 70 non‐sedated pre‐school children with and without recurrent wheeze. Cytokines (IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐12p70, IL‐13, TNF‐α) were measured in 50 μl samples using ultrasensitive multiplexed liquid bead array. The success rate of condensate collection increased from 64% (without recirculation) to 83% (after breath recirculation), and mean condensate volume from 214 to 465 μl respectively. Detection of cytokines was successful in 95–100% of samples. Cytokine concentrations before and after breath recirculation were not different (p > 0.232). In asthmatic children, only TNF‐α concentrations were significantly decreased, compared to non‐asthmatics. In pre‐school children, the collection of EBC is feasible using a new closed glass condenser with breath recirculation system. This new method may help to assess – non‐invasively – cytokine profiles in asthmatic and non‐asthmatic pre‐school children.