Premium
Suppressive effects of formoterol and salmeterol on eotaxin‐1 in bronchial epithelial cells
Author(s) -
Chu YuTe,
Chang TaiTsung,
Jong YuhJyh,
Kuo PoLin,
Lee HsiMing,
Lee MinSheng,
Chang HuiWen,
Hung ChihHsing
Publication year - 2010
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2009.00906.x
Subject(s) - formoterol , salmeterol , eotaxin , medicine , ccl11 , formoterol fumarate , western blot , chemokine , immunology , eosinophil , pharmacology , endocrinology , asthma , inflammation , chemistry , budesonide , biochemistry , gene
Chu Y‐T, Chang T‐T, Jong Y‐J, Kuo P‐L, Lee H‐M, Lee M‐S, Chang H‐W, Hung C‐H. Suppressive effects of formoterol and salmeterol on eotaxin‐1 in bronchial epithelial cells.
Pediatr Allergy Immunol 2010: 21: 345–352.
© 2009 John Wiley & Sons A/S Eotaxin‐1 (CCL11), an eosinophil‐specific C‐C chemokine, is a potent chemoattractant for mobilization of eosinophils into airways after allergic stimulation. Eotaxin‐1 recruits eosinophils into inflammatory sites, and may play a role in the pathogenesis of asthma. Formoterol and salmeterol are two inhaled long acting β 2 adrenoceptor agonists (LABAs), widely used for the local treatment of asthma. However, little is known about their effects on the eotaxin‐1 expression of bronchial epithelial cells. BEAS‐2B cells were stimulated by adding IL‐4 with or without 2 h pre‐treatment of formoterol or salmeterol. The protein and mRNA expression of eotaxin‐1 were measured by ELISA assay and real‐time PCR, respectively. Effects of formoterol and salmeterol on nuclear and cytosolic pSTAT‐6 expression were evaluated by Western blot and immunofluorescence study. Formoterol and salmeterol (10 −7 –10 −10 m ) significantly down‐regulated IL‐4‐ induced eotaxin‐1 expression in BEAS‐2B cells. A specific β 2 adrenoceptor antagonist (ICI 118,551) reversed their suppression of eotaxin‐1 production. Forskolin, an cAMP activator, could also suppress the expression of eotaxin‐1 by IL‐4 in a dose dependent manner (10 −7 –10 −10 m ). The western blot and immunofluorescence studies demonstrated that formoterol 10 −7 m suppressed the nuclear expression of pSTAT‐6. Formoterol and salmeterol, two inhaled long‐acting β 2 agonists, down‐regulated IL‐4‐ induced eotaxin‐1 expression in BEAS‐2B cells. The effect was mediated via the β 2 adrenoceptor, and cAMP. Formoterol significantly down‐regulated pSTAT6 at higher concentration, and further turned off the IL‐4 signaling pathway.