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Enhanced expression of IL‐27 mRNA in human newborns
Author(s) -
Krumbiegel Doreen,
AnthogalidisVoss Christina,
Markus Helena,
Zepp Fred,
Meyer Claudius U.
Publication year - 2008
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2007.00685.x
Subject(s) - messenger rna , secretion , receptor , medicine , cord blood , microbiology and biotechnology , immunology , biology , endocrinology , gene , biochemistry
Interleukin (IL)‐27, a heterodimer composed of Epstein–Barr virus‐induced gene 3 (EBI3) and p28, is an early product of activated dendritic cells (DC). Binding of IL‐27 to the WSX‐1 receptor initiates Th1 (Thelper 1) responses in naïve T cells. In order to assess the Th1 responses in human neonates with high susceptibility to infectious diseases, expression of EBI3‐, p28‐ and WSX‐1‐mRNA in response to Toll‐like receptor ligands was compared in neonate and adult monocyte‐derived (m)DC. Only the combined addition of ligands induced expression of IL‐27p28 mRNA. Surprisingly, neonatal mDC produced significantly more IL‐27p28 mRNA than that obtained from adults. Furthermore, there was enhanced expression of EBI3 mRNA in cord blood as compared with adult blood. In addition, the secretion of WSX‐1 mRNA in neonatal mDC and T cells was also significantly increased. Taken together, these findings indicate that the restricted Th1 responses in human newborns owing to deficient IL‐12 production may be compensated for, in part, by enhanced IL‐27 secretion.