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Cysteinyl‐leukotrienes in nasal lavage fluid in children with asthma
Author(s) -
Singer Florian,
Horak Friedrich,
Friesenbichler Waltraud,
Schneider Barbara,
Szépfalusi Zsolt,
Frischer Thomas
Publication year - 2008
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2007.00614.x
Subject(s) - medicine , asthma , exhaled breath condensate , gastroenterology , repeatability , pulmonary function testing , respiratory disease , immunology , lung , chemistry , chromatography
The aim of this study was to investigate repeatability of cysteinyl‐leukotrienes (cys‐LT) measurements in nasal lavage fluid (NLF) and to determine if cys‐LT levels in NLF are related to asthma severity in children. As a second outcome, we investigated if cys‐LT in NLF reflect lower airway inflammation as assessed by exhaled NO measurement. To assess the repeatability of cys‐LT measurements, two NLF samples were obtained from eight healthy controls 24 h apart. Sixty‐nine asthmatic children (mean age; range: 12.8; 7.3–17.7 yr), which were grouped according to asthma severity were studied cross‐sectionally on one occasion. Cys‐LT in NLF were analyzed using a specific enzyme immunoassay, exhaled NO, and pulmonary function parameters were measured. The coefficient of repeatability for the repeated cys‐LT measurements was 1.45 pg/ml. Cys‐LT levels in NLF differed significantly between asthma severity groups (p < 0.001): mild intermittent: [median (IQR)] 6.88 pg/ml (2.00–27.87); mild persistent: 21.09 pg/ml (4.50–84.67); and moderate persistent asthmatics: 36.41 pg/ml (11.03–118.40). Concentration of cys‐LT in NLF and exhaled NO was positively correlated (r = 0.85; p < 0.001). In conclusion, concentration of cys‐LT in NLF correlates with asthma severity in children and is related to lower airway inflammation.