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Gender‐limited association of cytotoxic T‐lymphocyte antigen‐4 (CTLA‐4) polymorphism with cord blood IgE levels
Author(s) -
Chang JenChieh,
Liu ChiehAn,
Chuang Hau,
Ou ChiaYu,
Hsu TeYao,
Huang EngYen,
Yang Kuender D.
Publication year - 2004
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.2004.00161.x
Subject(s) - medicine , immunology , cytotoxic t cell , cord blood , ctla 4 , antigen , immunoglobulin e , antibody , immune system , t cell , genetics , biology , in vitro
Allergic mechanism has long been attributed to IgE‐mediated reaction. The relationship between gene polymorphism and cord blood IgE (CB IgE) is unclear. We investigated whether elevation of CB IgE levels was associated with polymorphisms of cytotoxic T‐lymphocyte antigen 4 (CTLA‐4) at (−318) CT and (+49) AG positions in a gender‐limited fashion. CB IgE levels were determined by Pharmacia CAP system and the CTLA‐4 polymorphisms at (−318) and (+49) were determined by restriction fragment length polymorphism (RFLP). A total of 644 consecutive umbilical cord bloods were collected for this study. 32.9% of newborn infants had detectable IgE levels (≥0.35 kU/l). 25.6% of the male newborns had elevated CB IgE levels (≥0.5 kU/l) similar to those of the female newborns (22.7%). The CTLA‐4 polymorphism at (+49) but not (−318) was significantly associated with elevated CB IgE levels (p = 0.004). The association of CTLA‐4 (+49) A allele with elevated CB IgE levels was found only in females. Both male and female infants with different CTLA‐4 (−318) genotypes had no difference in the rates of elevated CB IgE levels. A linkage disequilibrium between CTLA‐4 (+49) G and (−318) C allele was found in this Chinese population. Subjects with the (+49, GG and −318, CC) genotype had a significantly lower rate of elevated CB IgE levels. Association of the CTLA‐4 (+49) polymorphism with elevated CB IgE levels was found only in female infants. Newborn infants with the (+49, GG and −318, CC) genotype tended to have a low rate of elevated CB IgE.