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Isolation of type 1 and type 2 cloned mite allergen‐specific T cells from an asthmatic child
Author(s) -
Chiang BorLuen,
Ding HanJen,
Chou ChenCheng,
Hsieh KueHsiung
Publication year - 1996
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.1996.tb00132.x
Subject(s) - immunology , allergen , medicine , house dust mite , pathogenesis , allergy , t cell , t helper cell , mite , asthma , biology , immune system , botany
Allergen‐specific T cells have been thought to play a central role in the pathogenesis of asthma. It has been well documented that allergen‐specific T cells derived from atopic patients are predominantly of type 2 T helper cell pattern. However, allergen‐specific T cells derived in nonatopic normals are of type 1 in contrast to alopic patients. The purpose of the study was to develop and characterize both mite allergen‐specific TH 1 and TH 2 clones from the same asthmatic child. With exogenous supplemental cytokine, both TH 1 and TH 2 clones from the same patient have been developed and maintained in this laboratory for more than one year. All these T cell clones showed dose dependent allergen‐specific proliferative response and expressed with CD4 + , CD45RA − markers. Elucidation of the origin and interaction between these two different types of T helper cells might shed light on understanding the pathogenesis of atopic diseases and the mechanisms of hyposensitization in atopic patients.