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Mucosal T cells recovered from mice after infection with respiratory syncytial virus display a memory/activation phenotype
Author(s) -
Kimpen J. L. L.,
Ogra P. L.
Publication year - 1995
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.1995.tb00270.x
Subject(s) - medicine , phenotype , virus , respiratory system , virology , immunology , pneumovirus , paramyxoviridae , viral disease , genetics , biology , gene
Pgp‐1 expression was studied as a marker of memory/activation on systemic and mucosal T cells of BALB/c and C57BL/6 mice after infection with respiratory syncytial virus (RSV), using two‐color dual fluorescence flow cytometry employing anti‐L3T4 (CD4), anti‐Ly2 (CD8), and anti‐Pgp‐1 (CD44) monoclonal antibodies. Pgp‐1 was expressed in relatively low densities on T cells of C57BL/6 mice, allowing differentiation of a dual population of Pgp‐1 10 and Pgp‐1 hi T cells after antigenic stimulation in vivo. On the contrary, T cells of BALB/c mice were uniformely Pgp‐1 hi , making this mouse strain less suitable for studies with this marker. In blood and spleen consistently more CD8 + than CD4 + T cells were Pgp‐1 hi , while in BAL more CD4 + than CD8 + T cells were Pgp‐1 hi After primary but not after secondary infection, CD4 + Pgp‐1 hi T cells increased significantly in the blood and spleen. After secondary infection both CD4 + Pgp‐1 hi and CD8 + Pgp‐1 hi T cells increased in the BAL. It is hypothesized that after primary infection systemic RSV‐specific T cells acquire an activation/memory phenotype as characterized by an enhanced expression of Pgp‐1, resulting in a faster and stronger influx of these cells in the lungs after secondary infection.