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Leukotriene B 4 and C 4 generation by blood leukocytes after ex vivo stimulation by Ca‐ionophore and opsonized zymosan in children with atopic dermatitis
Author(s) -
Shimizu T.,
Kristjansson S.,
Wennergren G.,
Strannegård I.L.,
Strandvik B.,
Shimizu Toshiaki
Publication year - 1994
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.1994.tb00224.x
Subject(s) - zymosan , stimulation , eosinophil , medicine , ex vivo , immunology , leukotriene , ionophore , endocrinology , in vivo , in vitro , biology , calcium , biochemistry , asthma , microbiology and biotechnology
The ex vivo release of leukotrienes B 4 (LTB 4 ) and C 4 (LTC 4 ) from the leukocytes of children with atopic dermatitis (AD) was evaluated after stimulation with Ca‐ionophore and opsonized zymosan and compared with that of control children of similar ages. The blood eosinophil counts and total serum IgE levels in AD children were significantly higher than those in control children. The production of LTC 4 , but not LTB 4 , was significantly higher in AD children than in control children. There was a significant correlation between the relative blood eosinophil count and LTC 4 generation after stimulation with both Ca‐ionophore and opsonized zymosan in all subjects. Calculations of the amount of LTC 4 produced per eosinophilic cell showed that there was no significant difference between cells from AD children and control children in terms of their ability to produce LTC 4 . These findings suggest that the enhanced LTC 4 generation is due to increased numbers of eosinophils rather than to enhanced releasability of these cells.