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Case report of common variable immunodeficiency with functional T‐helper defect and reduced CD4 + LAM −1 T cells
Author(s) -
Esser M.,
Bouic P.,
Nel N.,
Cooper R.,
Daniels J.
Publication year - 1991
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.1991.tb00206.x
Subject(s) - common variable immunodeficiency , immunology , cd8 , medicine , t lymphocyte , monoclonal antibody , antibody , immunodeficiency , cd3 , t cell , in vitro , microbiology and biotechnology , biology , antigen , immune system , biochemistry
A 10 year‐old boy presenting with an empyema and a previous medical history of recurrent and disseminated infections was investigated immunologically. The serum immunoelectrophoresis showed hypogammaglobulinaemia. The in vitro lymphocyte proliferation to T‐cell mitogens (PHA and Con A) was diminished and the response to a T‐dependent B cell mitogen (PWM) was severely diminished. Phenotypic analysis of his lymphocytes with monoclonal antibodies revealed normal CD3 + , CD4 + and CD8 + cells with a normal CD4: CD8 ratio. Double immunofluorescence with Leu 8 antibody revealed a selective deficiency of the CD4 + LAM 1 − subset. Co‐culture experiments between patient's B cells and supernatants from normal T cells showed that the patient's B cells could secrete Ig in vitro. We suggest that the Leu 8 monoclonal antibody may be useful in defining abnormalities in the regulatory subsets of T lymphocytes and that these abnormalities may be relevant in the pathogenesis of common variable immunodeficiency.