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Comparison of two IgE antibody tests with skin test and clinical history in asthmatic patients
Author(s) -
Warner J. A.,
Little S. A.,
Warner J. O.
Publication year - 1990
Publication title -
pediatric allergy and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.269
H-Index - 89
eISSN - 1399-3038
pISSN - 0905-6157
DOI - 10.1111/j.1399-3038.1990.tb00006.x
Subject(s) - immunoglobulin e , medicine , allergy , immunology , allergen , radioallergosorbent test , mast (botany) , alternaria , mite , asthma , antibody , mast cell , biology , botany
The multiple allergosorbent chemiluminescent assay (MAST‐CLA) is a system to measure total and allergen‐specific IgE in human serum by means of a chemiluminescent immuno‐enzymatic system. The test has been compared with skin test, RAST and clinical history in 67 atopic, asthmatic children. The individual percentage agreement between MAST‐CLA and skin test was grass pollen 67%, tree pollen 82%, cat 76%, dog 84%, house dust mite 87%, alternaria 64%, aspergillus 79%, cladosporium 84%, penicillium 93%, milk 78% and egg 76% and between MAST‐CLA and RAST was grass pollen 62%, tree pollen 72%, cat 75%, dog 72% and mite 87%. The total IgE levels on MAST‐CLA did not agree with PRIST results. MAST‐CLA was randomly duplicated and proved repToducible in 85% of tests. Changes between positive and negative results occurred in only 4% of tests. Clinical history predicted allergy diagnosis accurately in 21 (31. 5%) cases whilst MAST‐CLA provided additional information in 14 (21%). MAST‐CLA proved least reliable for grass pollen allergy diagnosis, which has prompted a change in allergen composition for this assay. MAST‐CLA is a simple in vitro test for specific IgE to 35 allergens which compares favourably with RAST. The variation in correlates with other techniques of allergy diagnosis, however, indicates that there are differences in credibility for each result within the multiple test system.