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Association studies of Toll‐like receptor gene polymorphisms with allograft survival in renal transplant recipients of North India
Author(s) -
Srivastava Priyanka,
Singh Anugya,
Kesarwani Praveen,
Jaiswal Praveen K.,
Singh Vibha,
Mittal Rama D.
Publication year - 2012
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/j.1399-0012.2011.01577.x
Subject(s) - medicine , transplantation , genotype , allele , tlr2 , kidney transplantation , immunology , organ transplantation , receptor , restriction fragment length polymorphism , tlr3 , toll like receptor , gastroenterology , gene , innate immune system , biology , genetics
Srivastava P, Singh A, Kesarwani P, Jaiswal PK, Singh V, Mittal RD. Association studies of Toll‐like receptor gene polymorphisms with allograft survival in renal transplant recipients of North India. Abstract: Organ transplantation itself inevitably activates the innate immune system by Toll‐like receptors (TLRs), potentially leading to allograft rejection and graft failure. We evaluated the possible association of TLR2, TLR3 , and TLR9 polymorphisms of donor‐recipient pairs and acute rejection in renal transplant patients of North India. TLR2 (−196 to −174 del), TLR3 (c.1377C/T; rs 3775290), and TLR9 (+2848 G/A; rs 352140) were genotyped using DNA samples from 200 donor‐recipient pairs of live donor kidney transplantation by applying Restriction Fragment Length Polymorphism (RFLP) methodology. The variant allele frequency of TLR2 (−196 to −174 del) was significantly different between recipients and donors (7.5% vs. 5.0%; p = 0.049; OR = 3.9; 95%CI = 1.01–15.32). However, no significant association for allograft rejection was observed in transplant recipients for TLR3 and TLR9 . Interestingly, a low prevalence of AA genotype of TLR9 + 2848 G>A was observed in rejecters when compared with non‐rejecters, demonstrating protective association with allograft rejection (OR = 0.30, 95% CI = 0.12–0.88, p = 0.028). An allele in patients was also observed to be associated with higher rejection‐free survival (log‐rank = 0.044). These TLR gene polymorphisms, upon further evaluation, may be helpful in elucidation of immunobiological mechanisms associated with renal graft rejection.