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Non‐HLA T‐cell reactivity during the first year after HLA‐identical living‐related kidney transplantation
Author(s) -
Gerrits Jeroen H.,
Van De Wetering Jacqueline,
Drabbels Jos J.,
IJzermans Jan N.M.,
Claas Frans H.J.,
Weimar Willem,
Van Besouw Nicole M.
Publication year - 2009
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/j.1399-0012.2009.01014.x
Subject(s) - elispot , transplantation , human leukocyte antigen , granzyme b , medicine , minor histocompatibility antigen , immunology , kidney transplantation , antigen , histocompatibility , peripheral blood mononuclear cell , major histocompatibility complex , cd8 , biology , in vitro , biochemistry
Background: It has been reported that donor‐reactive T‐cell responses may decrease during the first year after HLA‐mismatched organ transplantation. We wondered whether donor‐reactive T‐cell responses directed to minor histocompatibility antigens (mHAgs) or other non‐HLA antigens also decrease after HLA‐identical living‐related (LR) kidney transplantation. Methods: We studied donor‐reactive T‐cell responses by IFN‐γ and granzyme B (GrB) Elispot assays in 15 HLA‐identical LR kidney transplant recipients before, six months and one yr after transplantation. Third‐party reactivity was used as control. Patient and donor peripheral blood mononuclear cells were typed for 11 known mHAgs. Results: During the study period, 60% and 36% of the patients demonstrated donor‐reactive IFN‐γ and GrB producing cells (pc), respectively. The number of donor‐reactive IFN‐γ and GrB pc was significantly lower than the number of third‐party reactive IFN‐γ and GrB pc. After transplantation, donor‐reactivity and third‐party reactivity were comparable to pre‐transplant values. No relation was found in mHAg mismatches between donor and recipient and donor‐reactive T‐cell response. Conclusions: Donor‐reactivity could be detected before and after HLA‐identical LR kidney transplantation, but was not related with the number of mHAg mismatches, and did not decrease after transplantation.