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Protein levels of heme oxygenase‐1 during reperfusion in human kidney transplants with delayed graft function
Author(s) -
Öllinger Robert,
Kogler Pamela,
Biebl Matthias,
Sieb Michael,
Sucher Robert,
Bösmüller Claudia,
Troppmair Jakob,
Mark Walter,
Weiss Helmut,
Margreiter Raimund
Publication year - 2008
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/j.1399-0012.2008.00800.x
Subject(s) - heme oxygenase , medicine , kidney , heme , reperfusion injury , western blot , urology , renal function , pathological , ischemia , enzyme , biochemistry , biology , gene
Introduction: Delayed graft function (DGF) as a consequence of ischemia reperfusion injury (IRI) is associated with a decrease in long‐term allograft survival. Heme oxygenase‐1 (HO‐1) is a stress responsive gene that is highly expressed in multiple pathological processes. The aim of our study was to analyze whether HO‐1 protein levels in human kidney transplants during IRI correlate with the incidence of DGF. Methods: Kidney biopsies were obtained from 27 kidney allografts at two time points: at the end of cold storage and shortly after reperfusion. Samples were analyzed for HO‐1 protein levels by Western blot. Results: Heme oxygenase‐1 protein levels were significantly higher in post‐reperfusion biopsies (39.4 vs. 13.7 arbitrary units, p = 0.001). In pre‐reperfusion biopsies no association was observed between HO‐1 protein levels and DGF. In post‐reperfusion biopsies, higher levels of HO‐1 protein were measured in kidneys with DGF (53.7 vs. 36.2 arbitrary units, p = 0.064). DGF kidneys showed a significantly higher increase from pre‐ to post‐reperfusion in HO‐1 protein (42.0 vs. 18.7 arbitrary units, p = 0.025). Conclusion: Heme oxygenase‐1 protein levels shortly after allograft reperfusion are closely related with initial graft function. Assessment thereof may be considered a valuable tool to predict DGF.