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The role of preservation solution on acid–base regulation during machine perfusion of kidneys
Author(s) -
Baicu Simona C,
Taylor Michael J,
Brockbank Kelvin GM
Publication year - 2006
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/j.1399-0012.2005.00451.x
Subject(s) - machine perfusion , perfusion , transplantation , medicine , kidney , urology , hepes , ex vivo , biochemistry , in vitro , chemistry , liver transplantation
To meet the current clinical organ demand, efficient preservation methods and solutions are needed to increase the number of viable kidneys for transplantation. In the present study, the influence of perfusion solution buffering strength on renal pH dynamics and regulation mechanisms during kidney ex vivo preservation was determined. Porcine kidneys were hypothermically machine perfused for 72 h with either Unisol TM ‐UHK or Belzer‐Machine Perfusion solution, Belzer‐MP solution. Renal perfusate samples were periodically collected and biochemically analyzed. The UHK solution, a Hepes‐based solution (35 mM), provided a more efficient control of renal pH that, in turn, resulted in minor changes in the perfusate pH relative to baseline, in response to tissue CO 2 and HCO production. In the perfusate of Belzer‐MP kidney group a wider range of pH values were recorded and a pronounced pH reduction was seen in response to significant rises in pCO 2 and HCO concentrations. The Belzer‐MP solution, containing phosphate (25 mM) as its main buffer, and only 10 mM Hepes, had a greater buffering requirement to attenuate larger pH changes.