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Correlation between endothelin expression in early post‐transplant biopsy specimens and long‐term allograft function in living‐related renal transplantation
Author(s) -
Takeda Masashi,
Ishida Toshiro,
Ishimura Takeshi,
Fujisawa Masato
Publication year - 2006
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/j.1399-0012.2005.00434.x
Subject(s) - medicine , transplantation , biopsy , creatinine , kidney transplantation , kidney , staining , pathology , urology , renal function , immunohistochemistry , calcineurin , renal biopsy , gastroenterology
Objective: We investigated whether degree of immunohistochemically evident endothelin (ET) expression in early post‐transplant biopsy specimens could predict long‐term allograft function in living‐related renal transplantation. Methods: Allograft biopsy specimens obtained from 40 patients with living‐related transplants were studied. Cases with episodes of acute rejection or calcineurin inhibitor toxicity were excluded. We immunostained graft biopsy specimens obtained at pre‐transplantation (PRE) and at 3 months (3M) afterward with anti‐ET antibody. The number of stained tubular epithelial cells per 1000 tubular cells was defined as the staining index (SI). In the 21 patients whom we could assess at 3 yr (3Y) after transplantation, the correlation between ET expression and long‐term graft function was examined. Results: Anti‐ET antibody staining was appreciable in tubular epithelium but not in glomeruli. Tubular SI at PRE and at 3M were 10.6 ± 15.3 and 32.0 ± 35.6 (mean ± SD) respectively (p < 0.01). When patients were classified according to SI (group A, SI < 25; group B, SI > 25), declining ratio in creatinine clearance at 3Y after transplantation for groups A and B with respect was 21.8% ± 15.4% and 41.9% ± 21.6% (p < 0.05). Conclusion: High ET expression in early post‐transplantion, biopsy specimens was related to poor long‐term allograft function following living‐related renal transplantation.