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High‐resolution oligonucleotide array‐CGH applied to the detection and characterization of large rearrangements in the hereditary breast cancer gene BRCA1
Author(s) -
Rouleau E,
Lefol C,
Tozlu S,
Andrieu C,
Guy C,
Copigny F,
Nogues C,
Bieche I,
Lidereau R
Publication year - 2007
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.2007.00849.x
Subject(s) - breakpoint , oligonucleotide , biology , genetics , gene duplication , computational biology , gene , comparative genomic hybridization , polymerase chain reaction , point mutation , mutation , genome , chromosome
We have developed a new method for detecting and characterizing large rearrangements in the BRCA1 gene based on high‐resolution oligonucleotide array‐CGH technology. We designed a specific CGH array for the BRCA1 gene and its flanking regions. We then used this approach to analyze nine DNA samples known to contain large deletions and large duplications. When possible, the deleted or duplicated region was sequenced to identify the break point. All the large rearrangements were detected by the new method, and their size was estimated to be within 1–2 kb. This enabled us to develop a simple polymerase chain reaction screening test for other family members. A refined choice of oligonucleotides should improve the precision of the breakpoint determination. Finally, the high resolution of oligonucleotide array‐CGH should help to detect new large rearrangements missed by other current methods.