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Detection of dystrophin deletion carriers using FISH analysis
Author(s) -
Calvano S.,
Memeo E.,
Piemontese M. R.,
Melchionda S.,
Bisceglia L.,
Gasparini P.,
Zelante L.
Publication year - 1997
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1997.tb02509.x
Subject(s) - dystrophin , genetic counseling , duchenne muscular dystrophy , fluorescence in situ hybridization , fish <actinopterygii> , biology , genetics , gene , muscular dystrophy , microbiology and biotechnology , chromosome , fishery
The detection of carrier status in female relatives of Duchenne/Becker muscular dystrophy patients is not always possible and this poses a problem in genetic counseling. We have developed a simple method that can be used in families in which affected males are characterized by the presence of a deletion within the dystrophin gene. PCR fragments, corresponding to the deleted regions are used as fluorescent probes for hybridization of peripheral lymphocytes nuclei of female relatives. The results obtained clearly demonstrate the feasibility of this method for detecting female DMD/BMD carriers.