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Marker chromosome identification by micro‐FISH
Author(s) -
Engelen John J. M.,
Loots Wil J. G.,
Motoh Petra C. C.,
Moog Ute,
Hamers Guus J. H.,
Geraedts Joep P. M.
Publication year - 1996
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1996.tb03781.x
Subject(s) - marker chromosome , isochromosome , metaphase , biology , centromere , chromosome , microbiology and biotechnology , genetics , fish <actinopterygii> , small supernumerary marker chromosome , hybridization probe , chromosome 17 (human) , karyotype , dna , gene , fishery
Micro‐FISH was used to elucidate the chromosomal origin of marker chromosomes in three patients. Ten copies of marker chromosomes were collected with microneedles from GTG banded metaphases, transferred to a collecting drop and amplified by means of DOP‐PCR. The PCR products were labeled with biotin‐14‐dATP and used as FISH probes for hybridization to normal metaphase chromosomes and to metaphase chromosomes of the patients (reverse painting). With the generation of chromosome region‐specific painting probes by PCR amplification of microdissected DNA and subsequent FISH it was possible to identify the marker chromosomes in all patients. One marker appeared to be derived from the centromere region of the X‐chromosome and the proximal third of the long arm, one from the centromere region of chromosome 17 and one marker chromosome was identified as an isochromosome 18p.