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Identification of the valine 408 to methionine mutation in the LDL receptor in a Greek patient with homozygous familial hypercholesterolemia
Author(s) -
Schuster H.,
Manke C.,
Fischer J.,
Keller C.,
Wolfram G.,
Zöllnery N.
Publication year - 1995
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1995.tb04062.x
Subject(s) - genetics , haplotype , mutation , familial hypercholesterolemia , exon , valine , biology , genomic dna , restriction fragment length polymorphism , restriction site , allele , methionine , microbiology and biotechnology , gene , polymerase chain reaction , restriction enzyme , amino acid , endocrinology , cholesterol
We have identified the cytosine to thymine change in the codon for amino acid 408 which causes valine to be replaced by methionine in exon 9 of the LDL receptor gene in a 12‐year‐old Greek boy living in Germany, with homozygous familial hypercholesterolemia, by using polymerase chain reaction‐amplified genomic DNA and subsequent restriction digestion. Homozygosity was confirmed by direct DNA sequencing. The mutation was present in both his parents, and his brother, grandmother, uncle and cousin. Six restriction fragment length polymorphisms of the LDL receptor gene were used to determine the haplotype of the defective allele. The haplotype was different from the one reported earlier in African Afrikaners and from Holland. We conclude that the mutation in the Greek boy probably occurred as an independent mutation. Because the parents are from different areas in Greece, this mutation may be common in Greeks.