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A new simple and rapid dual assay for AFP and free β hCG in screening for Down syndrome
Author(s) -
NørgaardPedersen Bent,
Alfthan Henrik,
Arends Jørgen,
Høgdall Claus K.,
Larsen Severin Olesen,
Pettersson Kim,
Stenman UlfHåkan,
Salonen Riitta
Publication year - 1994
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1994.tb03980.x
Subject(s) - medicine , gonadotropin , endocrinology , andrology , chemistry , hormone
We have evaluated a simple and rapid 2‐step dual‐label assay (DELFIA®) for alphafetoprotein (AFP) and free β subunit of human gonadotropin (hCGβ) in second‐trimester screening for Down syndrome. Based on stored serum samples from 1059 normal control pregnancies and 72 cases of Down syndrome, we have found the mean Multiple of Median (MoM) for AFP and free hCGβ to be 0.70 and 2.31, respectively. This is slightly but not significantly better than the values for the separate assay for AFP (0.76 MoM) and for intact hCG (2.11 MoM). However, the dual assay is much simpler than the separate assays and therefore prospective comparison trials should be carried out.