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PCR analysis of a three‐allelic PvuII—RFLP at D4S127 closely linked to the Huntington disease locus
Author(s) -
Willoh Renate,
Zühlke Christine,
Gerdes Barbara,
Wiese Stefan,
Thies Ulrike
Publication year - 1993
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1993.tb03828.x
Subject(s) - locus (genetics) , allele , genetics , genotyping , restriction fragment length polymorphism , biology , huntington's disease , southern blot , gene , genotype , microbiology and biotechnology , disease , medicine
Willoh R, Zühlke C, Gerdes B, Wiese S, Thies U. PCR analysis of a three‐allelic PvuII—RFLP at D4S127 closely linked to the Huntington disease locus. Clin Genet 1993: 43: 321–323. © Munksgaard, 1993 The polymorphic probe BJ56PS18 defined the genetic locus D4S127 closely linked to the Huntington disease (HD) gene. BJ56PS18 detects a three‐allelic polymorphism after PvuII digestion in Southern blots. For rapid genotyping in HD‐families, two different PCR conditions were established.