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Deciphering the fluorescent variability of human genomic heterochromatin by DA/DAPI technique
Author(s) -
Verma Ram S.,
Conte Robert A.,
Luke Sunay,
Sindwani Veena,
Macera Michael J.
Publication year - 1992
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1992.tb03253.x
Subject(s) - dapi , heterochromatin , stain , biology , staining , fluorescence , genetics , microbiology and biotechnology , chromosome , gene , physics , quantum mechanics
A number of selective staining techniques have been utilized to decipher the variability of pericentromeric heterochromatin. One such technique is called DA/DAPI and it is believed to be stain specific. However, we demonstrate otherwise and suggest that pericentromeric regions of all human chromosomes stain positive by DA/DAPI‐technique. It must be emphasized that the incidence of DA/DAPI positive stained chromosomes, other than 1, 9, 15, 16 and Y, is a rare occurrence and only a small portion of the pericentromeric region is DA/DAPI positive, as reported here using 50 normal individuals.