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Nucleotide sequence and PCR‐amplification of a polymorphic Mbol site in human DNA marker D4S95 linked to the Huntington disease locus
Author(s) -
Nørremølle Anne,
Sørensen Sven Asger,
Arctander Peter
Publication year - 1992
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1992.tb03240.x
Subject(s) - locus (genetics) , genetics , biology , genotype , polymerase chain reaction , genetic marker , microbiology and biotechnology , nucleic acid sequence , dna , nucleotide , dna sequencing , gene
The nucleotide sequence of the probe BS674E‐D defining the genetic locus D4S95 was assessed, and used for designing PCR‐primers amplifying a 175 bp fragment containing a polymorphic Mbol site. With the described conditions it is now possible to use PCR followed by digestion for rapid determination of genotype in this marker system.