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In situ fluorescence hybridization of Y translations: cytogenetic analysis using probes Y190 and Y431
Author(s) -
Kozma Robert,
Adinolfi Matted
Publication year - 1988
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1988.tb03431.x
Subject(s) - biology , microbiology and biotechnology , sexing , chromosomal translocation , hybridization probe , fluorescence in situ hybridization , in situ hybridization , metaphase , interphase , chromosome , cytogenetics , autosome , genetics , dna , gene , gene expression
Two moderately repetitive DNA probes (Y190 and Y431) and a fluorescent in situ hybridization technique, using a biotin, avidin, anti‐avidin system, were employed to investigate a group of patients with Y chromosome abnormalities. In normal male subjects, a bright fluorescent spot could be detected in cells in interphase and on the short arm of the Y chromosome in metaphase spreads. Translocations of DNA fragments of the short arm of the Y chromosome to autosomes 10, 13 and 15 were observed in five patients. In a 45,XX male subject the translocation involved one of the X chromosomes. With this in situ hybridization procedure, bright fluorescent spots were also noticed in uncultured amniotic cells and chorionic cellular elements from male fetuses, thus allowing a rapid and reproducible approach to prenatal fetal sexing.