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Convoluted cells as a marker for maternal cell contamination in CVS cultures
Author(s) -
Hertz J. M.,
Jensen P. K. A.,
Therkelsen A. J.
Publication year - 1987
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1987.tb02834.x
Subject(s) - biology , placenta , microbiology and biotechnology , cell culture , andrology , distal convoluted tubule , pregnancy , fetus , genetics , medicine , nephron , kidney
In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X‐chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.