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Unspecific reactions of HLA‐B antisera on fibroblasts from patients and carriers of Duchenne muscular dystrophy
Author(s) -
Kirbach E. D. Aragon de,
Willers I.,
Singh S.,
Goedde H. W.
Publication year - 1986
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1986.tb00506.x
Subject(s) - fibroblast , human leukocyte antigen , duchenne muscular dystrophy , antiserum , cytotoxicity , antigen , muscular dystrophy , centrifugation , microbiology and biotechnology , monolayer , cell culture , immunology , chemistry , biology , in vitro , biochemistry , genetics
Skin fibroblasts of patients with Duchenne muscular dystrophy were analysed for HLA tissue antigens. For the examination of the antigens two modified methods were used: the microdro‐plet cytotoxicity assay on fibroblast suspension and the cytotoxicity assay on fibroblast monolayer cultures using fTuorochromasia. The HLA determination on the fibroblast suspensions of the patients showed a variety of unspecific HLA expressions which were not found in fibroblasts of healthy controls. These reactions were not apparent in the test in monolayer cultures. The exact cause has not been elucidated yet, but it appears to have a strong relation to some membrane abnormality in cells of DMD patients. The reproducible lack of unspecific reactions on monolayer cultures lead us to propose that these expressions arise from artificial injuries to membranes of these patient cells during trypsination or centrifugation. The validity of this test has been confirmed by a blind study.