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In vitro reversal of fragile‐X expression by exogenous thymidine
Author(s) -
Gardiner Gale B.,
Wenger Sharon L.,
Steele Mark W.
Publication year - 1984
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1984.tb00475.x
Subject(s) - thymidine , in vitro , biology , fragile x syndrome , microbiology and biotechnology , genetics
A virtually thymidine free medium was used to test the effects of exogenous thymidine on lymphocyte expression of the fragile‐X. De novo pathway thymidylic acid synthesis in the cells was blocked by FUdR. Our results suggest that in vitro exogenous thymidine is directly responsible for suppressing expression of the fragile‐X. More importantly, delayed addition of exogenous thymidine can negate fragile‐X expression after it has first been induced by FUdR.