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Quantitative immunoassays for diagnosis and carrier detection in cystic fibrosis
Author(s) -
Bullock Sarah,
Hayward Caroline,
Manson Jean,
Brock David J. H.,
Raeburn J. A.
Publication year - 1982
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1982.tb01380.x
Subject(s) - immunoradiometric assay , cystic fibrosis , immunoprecipitation , antiserum , heterozygote advantage , radioimmunoassay , antibody , microbiology and biotechnology , medicine , immunology , biology , genetics , gene , genotype
Quantitative immunoprecipitation and immunoradiometric assays have been developed for a protein present in the serum of cystic fibrosis homozygotes, and to a lesser extent in the serum of heterozygotes. When tested on a panel of sera from 14 cystic fibrosis patients, 29 heterozygotes and 23 controls, the immunoprecipitation assay allowed correct assignments to be made on 94% of occasions with one batch of antiserum and 95% with another. With the same panel of sera, the immunoradiometric assay allowed 94% correct assignments. It is suggested that such accuracy is the maximum that can be expected in the present state of knowledge of cystic fibrosis.