Premium
The apoE polymorphism studied by two‐dimensional, high‐resolution gel electrophoresis of serum
Author(s) -
BØRRESEN A.L.,
BERG K.
Publication year - 1981
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1981.tb01055.x
Subject(s) - polymorphism (computer science) , high resolution , apolipoprotein e , genetics , gel electrophoresis , electrophoresis , biology , genotype , medicine , gene , geography , disease , remote sensing
The apoE polymorphism of human very low density lipoprotein (VLDL) was studied by the two‐dimensional high‐resolution gel electrophoresis technique of O'Farrell, which combines isoelectric focusing and SDS‐polyacrylamide gel electrophoresis. With whole serum, six major patterns and two “sub‐variants” of apoE were observed. A series of twin pairs (21 monozygotic (MZ) and 13 dizygotic (DZ) pairs) as well as unrelated people were analyzed. Both members of MZ pairs always exhibited the same pattern, whereas DZ twins were often discordant. The patterns observed would be consistent with the hypothesis that three apoE isopeptides are coded for by three different alleles at one single locus. In this small series, all three postulated homozygous patterns, namely apoE‐II, apoE‐III and apoE‐IV as well as the three heterozygous patterns apoE‐11, 111; apoE‐III, IV and apoE‐ll, IV were seen.