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Polymorphism of apolipoprotein E
Author(s) -
Utermann G.,
Albrecht G.,
Steinmetz A.
Publication year - 1978
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1978.tb02101.x
Subject(s) - apolipoprotein b , phenotype , apolipoprotein e , polymorphism (computer science) , population , genetics , ultracentrifuge , biology , microbiology and biotechnology , allele , medicine , biochemistry , disease , gene , cholesterol , environmental health
Two methods for phenotyping apolipiprotein E are compared. One is based on preparation of VLDL by conventional ultracentrifugation, whereas the other uses heparin/Mg precipitation of VLDL. In principle, the same results were obtained by both methods. However, the group limits for the three different phenotypes Apo E‐N, Apo E‐ND and Apo E‐D were slightly different by the two methods. Phenotype Apo E‐D ‐ the phenotype characterizing type III dyslipoproteinemia ‐ is clearly definable by both methods. Hence the precipitation‐I.E.F. method for Apo E phenotyping provides a simple tool for genetic and population genetic studies and also for the routine diagnosis of hyperlipoproteinemia type III, based on the only specific marker known for this disease.