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Cultured fucosidosis fibroblasts: A simple technique demonstrating storage of tritiated‐fucose labeled material
Author(s) -
Wood Stephen
Publication year - 1976
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1976.tb00031.x
Subject(s) - fucose , chemistry , fibroblast , biochemistry , incubation , chromatography , in vitro , glycoprotein
Incorporation of tritiated fucose into the acid‐soluble and acid‐insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10‐day period. Fucosidosis cells incorporated significantly more label into the acid‐soluble, but not the acid‐insoluble fraction, than control cells. The ratio of acid‐soluble to acid‐insoluble radioactivity increased linearly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.