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Cultivated cells from diagnostic amniocentesis in second trimester pregnancies
Author(s) -
Hoehn Holger,
Bryant Eileen M.,
Karp Lawrence E.,
Martin George M.
Publication year - 1975
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1975.tb00359.x
Subject(s) - trypsinization , amniocentesis , amniotic fluid , aneuploidy , prenatal diagnosis , biology , andrology , second trimester , incidence (geometry) , fetus , pregnancy , medicine , genetics , chromosome , trypsin , biochemistry , physics , gene , optics , enzyme
From a total of 418 primary amniotic fluid colonies, 5.5 % were fibroblast‐like (F), 33.7 % epithelioid (E) and 60.8 % had characteristics of what was previously shown to be the principal class of clonable amniotic fluid cells (AF). Polyploidy occurred in all three categories, although both pure tetraploidy and mixoploidy were more frequent in E colonies. The incidence of non‐constitutional chromosomal changes was identical in AF and E type colonies if primary spreads were analyzed in situ without prior trypsinization. Spreads from pooled cell suspensions showed higher base‐line levels of both aneuploidy and structural changes. Analysis of individual colonies employing an in situ preparative technique is clearly the method of choice for a reliable cytogenetic prenatal diagnosis within the shortest possible period of time.