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In vitro autoradiographic studies of DNA synthesis in human testis with spermatogenic arrest
Author(s) -
Frajese Gaetano,
Santiemma Vittorio,
Re Massimo,
Bellacicco Antonio,
Fabbrini Aldo,
Fraccaro Marco
Publication year - 1974
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1974.tb02263.x
Subject(s) - dna synthesis , biology , in vitro , dna , medicine , andrology , endocrinology , microbiology and biotechnology , genetics
The uptake of 3 H‐thymidine by spermatogonia and resting spermatocytes was studied by autoradiography in human testis biopsies incubated in vitro from four groups of subjects: normal, prepuberal (hypogonadotropic hypogonadism), with spermatocytic arrest and with spermatidic arrest. In the prepuberal group there was a very low DNA synthesis rate. In the spermatidic arrest group the number of DNA synthesizing cells was higher than that of the normal control group while in the spermatocytic arrest group it was lower. These results indicate a possible different pathogenesis for the two forms of spermatogenic arrest. In the spermatocytic arrest there is a primary deficiency of germ cells synthesizing DNA, while in the spermatidic arrest, synthesis is active and the arrest is probably due to an enzymatic (?) metabolic block.