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Lysosomal enzyme levels in human amniotic fluid cells in tissue culture: II. α‐galactosidase, β‐galactosidase and α‐arabinosidase
Author(s) -
Sutherland G. R.,
Butterworth J.,
Broadhead D. M.,
Bain A. D.
Publication year - 1974
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1974.tb01705.x
Subject(s) - amniotic fluid , enzyme , enzyme assay , subculture (biology) , biology , andrology , beta galactosidase , fetus , endocrinology , medicine , microbiology and biotechnology , biochemistry , pregnancy , gene expression , genetics , gene
Changes in the activities of α‐galactosidase, β‐galactosidase and a‐arabinosidase in amniotic fluid cells with time in culture were studied. Marked fluctuations in all three enzymes occurred with passage. In certain cell strains, β‐galactosidase showed a marked rise in activity correlated with passage. The activity of all three enzymes, in amniotic fluid cells at the third passage, was correlated with the total time taken to reach con‐fluency. There was no consistent pattern of enzyme activity associated with the time after subculture. Enzyme levels in cell strains derived from serial samples of amniotic fluid from several women showed large differences in activity unrelated to gestational age.