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Cystic fibrosis: Synthesis of ciliary inhibitor by amniotic cells
Author(s) -
Bowman Barbara H.,
Lockhart Lillian H.,
Herzberg Victoria L.,
Barnett Don R.,
Armstrong Donald,
Kramer John
Publication year - 1973
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1973.tb01933.x
Subject(s) - cystic fibrosis , fetus , cilium , amniotic fluid , fibroblast , andrology , biology , endocrinology , medicine , cell culture , pathology , microbiology and biotechnology , pregnancy , genetics
The presence of a ciliary inhibitor in media of cultured amniotic cells obtained from a fetus heterozygous for cystic fibrosis has been observed by the oyster gill cilia assay. The chromatographic fraction containing the inhibitor corresponded to eluted fractions chromatographed from cystic fibrosis fibroblast media and serum. An analogous chromatographic fraction from media of cultured amniotic cells from two proportedly normal fetuses did not inhibit cilia. The chromatographic fraction from media of cultured amniotic cells of a fetus at high risk for cystic fibrosis did not inhibit ciliary activity. Serum was collected from this baby seven weeks after birth and also did not inhibit ciliary action, indicating a homozygous normal genotype. These observations may lead to the development of an antenatal test for cystic fibrosis.