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Giemsa banding patterns of human chromosomes *
Author(s) -
Crossen Peter E.
Publication year - 1972
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1972.tb01455.x
Subject(s) - giemsa stain , g banding , biology , karyotype , genetics , staining , microbiology and biotechnology , chromosome , gene
A method of obtaining differentially stained chromosomes is described. The method based on the treatment of the chromosomes with NaOH and subsequent incubation in phosphate buffer and Giemsa staining gave distinct banding patterns in both blood and bone marrow chromosomes. The banding patterns in some instances agreed closely with those of other methods but in others they were dissimilar. A probable explanation for the differences in banding patterns is the various treatments involved in producing banded chromosomes. While repetitive DNA may play a part in band formation, other factors may also be important.