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Identification of D group chromosomes by autoradiography and fluorescence microscopy
Author(s) -
Krmpotic Eva,
Szego Katarina,
Modestas Reoina
Publication year - 1971
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1971.tb00298.x
Subject(s) - fluorescence microscope , microscopy , fluorescence , staining , fluorescence in situ hybridization , fluorescent staining , biology , microbiology and biotechnology , chromosome , pathology , genetics , medicine , optics , gene , physics
Identification of D group chromosomes was done by quinacrine mustard staining and fluorescence microscopy, as well as by 3 H–thymidine labeling and autoradiography. No special instruments were used for the fluorescence microscopy studies. Chromosome 13 was recognized easily by fluorescence microscopy by its typical fluorescence pattern in the lower two–thirds of the long arm, the region of the late labeling in autoradiography. Chromosomes 14 and 15 could be recognized by fluorescence microscopy in most cases. If differentiation is not quite definite, 3 H–thymidine labeling and autoradiography should be used to support the findings.