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Studies of human chromosomes by DNA‐binding fluorochromes I. The normal chromosome complement
Author(s) -
Hagen Carl Birger,
Berg Kåre
Publication year - 1970
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1970.tb02247.x
Subject(s) - metaphase , karyotype , biology , chromosome , fluorescence , microbiology and biotechnology , dna , genetics , labelling , biochemistry , gene , physics , quantum mechanics
Caspersson's method of labelling chromosomes with DNA‐binding fluorescent agents has been applied to the study of human chromosomes. Fluorescence distribution curves of normal metaphase chromosomes treated with quinacrine mustard (QM) were obtained by scanning transparent pictures of the labelled chromosomes in a Beckman Analytrol, an instrument normally used for scanning electrophoresis strips. Representative fluorescence distribution curves of the different chromosomes, as well as one complete “QM karyotype”, have been presented. The distribution curves of individual chromosomes appear to be characteristic and reproducible and it was concluded that the technique of fluorescent labelling holds great promise for identification of individual human chromosomes and chromosomal regions.