Premium
Component‐resolved in vitro diagnosis of carrot allergy in three different regions of E urope
Author(s) -
BallmerWeber B. K.,
Skamstrup Hansen K.,
Sastre J.,
Andersson K.,
Bätscher I.,
Östling J.,
Dahl L.,
Hanschmann K.M.,
Holzhauser T.,
Poulsen L. K.,
Lidholm J.,
Vieths S.
Publication year - 2012
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2012.02827.x
Subject(s) - daucus carota , allergen , allergy , sensitization , recombinant dna , pollen , medicine , immunology , chemistry , biology , horticulture , botany , biochemistry , gene
Background Carrot is a frequent cause of food allergy in E urope. The objective of this study was to evaluate a panel of carrot allergens for diagnosis of carrot allergy in S pain, S witzerland and D enmark. Methods Forty‐nine carrot allergic patients, 71 pollen allergic but carrot‐tolerant patients and 63 nonatopic controls were included. Serum Ig E to carrot extract, recombinant carrot allergens (r D au c 1.0104; r D au c 1.0201; r D au c 4; the isoflavone reductase‐like proteins r D au c I FR 1, r D au c IFR 2; the carrot cyclophilin r D au c C yc) were analyzed by I mmuno CAP . Results The sensitivity of the carrot extract‐based test was 82%. Use of the recombinant allergens increased the sensitivity to 90%. The D au c 1 isoforms were major allergens for S wiss and D anish carrot allergic patients, the profilin r D au c 4 for the S panish patients. The r D au c IFR 1 and r D au c IFR 2 were recognized by 6% and 20% of the carrot allergics, but did not contribute to a further increase of sensitivity. Among pollen allergic controls, 34% had Ig E to carrot extract, 18% to each of r D au c 1.0104, r D au c 1.0201 and r D au c 4, 8% to r D au c IFR 1 and 7% to r D au c IFR 2. Sensitization to r D au c C yc occurred in one carrot allergic patient and one nonatopic control. Conclusion Component‐resolved in vitro analyses revealed a significant difference in Ig E sensitization pattern between geographical regions and in the prevalence of sensitization to carrot components between carrot allergic and carrot‐tolerant but pollen sensitized patients.