Premium
Tropomyosin IgE‐positive results are a good predictor of shrimp allergy
Author(s) -
Gámez C.,
SánchezGarcía S.,
Ibáñez M. D.,
López R.,
Aguado E.,
López E.,
Sastre B.,
Sastre J.,
del Pozo V.
Publication year - 2011
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2011.02663.x
Subject(s) - shrimp , immunoglobulin e , allergy , allergen , immunology , medicine , food allergy , biology , antibody , fishery
To cite this article: Gámez C, Sánchez‐García S, Ibáñez MD, López R, Aguado E, López E, Sastre B, Sastre J, del Pozo V. Tropomyosin IgE‐positive results are a good predictor of shrimp allergy. Allergy 2011; 66 : 1375–1383. Abstract Background: Shrimp is a common cause of food allergy. Our aims were to determine the value of IgE antibodies in the diagnosis of shrimp allergy and to study red shrimp ( Solenocera melantho ) tropomyosin both as a new allergen and as a cross‐reactive IgE‐binding protein. Methods: We have studied 45 subjects. Skin prick test (SPT) was carried out in all subjects, and specific IgE (sIgE) to shrimp, recombinant and natural shrimp tropomyosins rPen a 1 and nPen m 1, recombinant Der p 10, and Dermatophagoides pteronyssinus was assessed by fluoroimmunoassay and/or immunoblotting. Double‐blind, placebo‐controlled food challenges were carried out to confirm diagnosis of shrimp allergy. Also, in vitro inhibition tests were performed to evaluate cross‐reactivity. Results: Shrimp allergy was confirmed in 18 shrimp‐allergic patients. Skin prick test and IgE antibodies to shrimp were positive in all shrimp‐allergic patients; sIgE to rPen a 1 was detected in 98% of these patients. Of the 18 shrimp‐tolerant patients, 61% had positive SPT to shrimp, 55% were IgE‐positive to shrimp, and 33% showed IgE antibodies to rPen a 1. Determination of IgE to rPen a 1 yielded a positive predictive value of 0.72 and a negative predictive value of 0.91. Conclusion: IgE levels to rPen a 1 provided additional value to the diagnosis of shrimp allergy. Some allergens in mite extract are recognized by patients who are allergic to shrimp, though their clinical relevance remains unknown.