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Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T‐regulatory responses upon systemic sensitization
Author(s) -
Schwarzer M.,
Repa A.,
Daniel C.,
Schabussova I.,
Hrncir T.,
Pot B.,
Stepankova R.,
Hudcovic T.,
Pollak A.,
TlaskalovaHogenova H.,
Wiedermann U.,
Kozakova H.
Publication year - 2011
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2010.02488.x
Subject(s) - immunology , immune system , biology , lactobacillus plantarum , spleen , allergy , sensitization , microbiology and biotechnology , bacteria , lactic acid , genetics
To cite this article: Schwarzer M, Repa A, Daniel C, Schabussova I, Hrncir T, Pot B, Stepankova R, Hudcovic T, Pollak A, Tlaskalova‐Hogenova H, Wiedermann U, Kozakova H. Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T‐regulatory responses upon systemic sensitization. Allergy 2011; 66 : 368–375. Abstract Background: The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen‐defined allergy prevention/treatment. Interventions with LAB are of increasing interest early in life when immune programming is initiated. Here, we investigated the effect of neonatal colonization with a recombinant LAB producing the major birch pollen allergen Bet v 1 in a murine model of type I allergy. Methods: We constructed a recombinant Lactobacillus ( L. ) plantarum NCIMB8826 strain constitutively producing Bet v 1 to be used for natural mother‐to‐offspring mono‐colonization of germ‐free BALB/c mice. Allergen‐specific immunomodulatory effects of the colonization on humoral and cellular immune responses were investigated prior and after sensitization to Bet v 1. Results: Mono‐colonization with the Bet v 1 producing L. plantarum induced a Th1‐biased immune response at the cellular level, evident in IFN‐γ production of splenocytes upon stimulation with Bet v 1. After sensitization with Bet v 1 these mice displayed suppressed IL‐4 and IL‐5 production in spleen and mesenteric lymph node cell cultures as well as decreased allergen‐specific antibody responses (IgG1, IgG2a, and IgE) in sera. This suppression was associated with a significant up‐regulation of the regulatory marker Foxp3 at the mRNA level in the spleen cells. Conclusion: Intervention at birth with a live recombinant L. plantarum producing a clinically relevant allergen reduces experimental allergy and might therefore become an effective strategy for early intervention against the onset of allergic diseases.