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Association of prostaglandin‐endoperoxide synthase 2 gene polymorphisms with asthma and atopy in Chinese children
Author(s) -
Chan I. H. S.,
Tang N. L. S.,
Leung T. F.,
Ma S. L.,
Zhang Y. P.,
Wong G. W. K.,
Wong C. K.,
Lam C. W. K.
Publication year - 2007
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2007.01400.x
Subject(s) - atopy , single nucleotide polymorphism , asthma , haplotype , allele , immunoglobulin e , medicine , immunology , genotype , genetics , biology , gene , antibody
Background: Cyclooxygenase‐2 (COX‐2) plays essential roles in inflammation. Previous studies have suggested associations between prostaglandin‐endoperoxide synthase 2 ( PTGS2 ) polymorphisms and prostaglandins production in asthma. Objective: We have investigated the effects of Chinese tagging single nucleotide polymorphisms (SNPs) of PTGS2 on asthma traits in 299 Chinese asthmatic children and 175 controls. Methods: Plasma total and allergen‐specific IgE were measured by enzyme immunoassay. PTGS2 .8473T→C in the 3′‐untranslated region of exon 10 and three tag SNPs covering most of the variations in PTGS2 haplotypes in Chinese were genotyped by restriction fragment length polymorphism. Results: Among the four SNPs, only PTGS2 .8473 showed significant association with asthma ( P = 0.034) and atopy ( P = 0.005 when compared with non‐atopic controls; P = 0.023 with all controls). Carriers of the C allele had a 1.5‐fold (95% confidence interval: 1.01–2.30) risk of developing asthma than those homozygous for the T allele. Multivariate regression revealed significant correlations between PTGS2 .8473 and forced expiratory volume in 1 s (FEV 1 ; P = 0.002) and peak expiratory flow rate (PEFR; P = 0.001) with age and gender adjusted. Patients with the C allele of PTGS2 .8473 had significantly lower FEV 1 (median: 90.0% vs 98.0%; P = 0.0047) and PEFR (70.0% vs 73.5%; P = 0.0065) than those homozygous for the T allele. No significant association between plasma total and allergen‐specific IgE and these SNPs or with their haplotypes was found. Conclusions: PTGS2 .8473 polymorphism is associated with asthma, atopy and lung function but not plasma IgE in Chinese children. This may help to explore the pharmacogenetics of COX‐2 inhibitors.