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Mala s 12 is a major allergen in patients with atopic eczema and has sequence similarities to the GMC oxidoreductase family *
Author(s) -
Zargari A.,
Selander C.,
Rasool O.,
Ghanem M.,
Gadda G.,
Crameri R.,
Scheynius A.
Publication year - 2007
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2006.01291.x
Subject(s) - allergen , immunoglobulin e , oxidoreductase , biology , recombinant dna , malassezia , flavin adenine dinucleotide , biochemistry , allergy , microbiology and biotechnology , chemistry , immunology , enzyme , antibody , gene , cofactor
Background: Atopic eczema (AE) is a chronic inflammatory skin disorder, characterized by impaired skin barrier and itch. The yeast Malassezia belongs to the normal human skin microflora and can induce IgE‐ and T‐cell‐mediated allergic reactions in AE patients. Previously, we have identified several IgE‐binding components in Malassezia sympodialis extract. Methods: Here, we report cloning, production and characterization of a M. sympodialis 67‐kDa allergen. Results: The sequence of the 67‐kDa protein, termed Mala s 12, showed sequence similarity to the glucose–methanol–choline (GMC) oxidoreductase enzyme superfamily and was expressed as a recombinant protein in Escherichia coli . The purified protein bound flavin adenine dinucleotide with 1:1 stoichiometry per monomer of protein. The protein‐bound flavin showed an extinction coefficient at 451 nm of 11.3 mM −1 cm −1 . The recombinant 67‐kDa protein did not show any enzymatic activity when tested as oxidase or dehydrogenase using choline, glucose, myo‐inositol, methanol, ethanol, 1‐pentanol, benzyl alcohol, 2‐phenylethanol, cholesterol or lauryl alcohol as possible substrates. Recombinant Mala s 12 was recognized by serum IgE from 13 of 21 (62%) M. sympodialis ‐sensitized AE patients indicating that the 67‐kDa component is a major allergen. Conclusions: The data show that Mala s 12 has sequence similarity to the GMC oxidoreductase family and is a major allergen in AE patients.