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Relevance of the determination of serum‐specific IgE antibodies in the diagnosis of immediate β ‐lactam allergy
Author(s) -
Fontaine C.,
Mayorga C.,
Bousquet P. J.,
Arnoux B.,
Torres M.J.,
Blanca M.,
Demoly P.
Publication year - 2007
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2006.01268.x
Subject(s) - immunoglobulin e , medicine , radioallergosorbent test , immunology , anaphylaxis , allergy , provocation test , antibody , drug allergy , pathology , alternative medicine
Background: Allergic reactions to β ‐lactams are the most frequent cause of adverse drug reactions mediated by specific immunologic mechanisms. They can be explored by in vivo and/or in vitro tests. The measurement of serum‐specific immunoglobulin E (IgE) presents several advantages: safety, simplicity, and availability to nonallergologist physicians. Objectives: To establish the diagnostic value of specific IgE determination in the diagnosis procedure of immediate β ‐lactam allergy. Methods: The in vitro determination of β ‐lactam‐specific IgE antibodies was compared in three well‐defined groups of patients ( n = 45): one with negative skin tests and a positive drug provocation test, another with positive skin tests, and a third control exposed population with good tolerance. Two techniques were used: the CAP‐FEIA system (Phadia ® ) commercially available and a homemade radioallergosorbent test (RAST). Results: The specificity of CAP‐FEIA ranged from 83.3% to 100% and sensitivity from 0% to 25% depending on initial clinical manifestations. The specificity of RAST was between 66.7% and 83.3% and sensitivity 42.9% and 75%. In the subgroup of patients with an anaphylactic shock and negative skin tests, the sensitivity and specificity of RAST were 75%. Positive and negative predictive values were 45.5% and 77.1% with CAP‐FEIA and 38.5% and 81.5% with RAST, respectively. Conclusion: These results indicate that, although the specificity of β ‐lactam‐specific IgE measurement is good, sensitivity is low. Immunoglobulin E measurement should be limited to patients with a clinical history of anaphylactic shock and negative skin tests in order to avoid a drug provocation test. More sensitive assays should be developed.