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Identification of tryptase‐ and chymase‐related gene clusters in human mast cells using microarrays
Author(s) -
Dahl C.,
Saito H.,
Kruhøffer M.,
Schiøtz P. O.
Publication year - 2006
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2005.00950.x
Subject(s) - chymase , tryptase , mast cell , biology , microbiology and biotechnology , gene expression , gene , dna microarray , immunology , gene expression profiling , genetics
Tryptase and chymase are the two major granular proteases present in human mast cell (MC)s. We used oligonucleotide microarray to measure the levels of approximately 22 000 transcripts in cord blood‐derived MCs at 4 weeks, 8 weeks, 12 weeks and 18 weeks in culture. Tryptase ( TPSB2 ) was expressed at the highest level among all transcripts and its expression level reached a plateau at 8 weeks. On the other hand, the expression level of chymase ( CMAI ) doubled every 4–6 weeks. A similar tendency was found at the protein levels with FACS analysis. After filtering the transcripts with MC‐specificity, hierarchical clustering analysis identified 494 and 81 transcripts in the same clusters with tryptase and chymase, respectively. MC‐specific genes, KIT and HDC were found in the tryptase cluster. In the chymase cluster, a critical suppressor for cell senescence, BMI1 and the several related genes were found, suggesting that chymase expression may be closely related to cell senescence/quiescence events.