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High sensitivity of basophils predicts side‐effects in venom immunotherapy
Author(s) -
Kosnik M.,
Silar M.,
Bajrovic N.,
Music E.,
Korosec P.
Publication year - 2005
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2005.00894.x
Subject(s) - cd63 , basophil activation , medicine , venom , basophil , immunology , side effect (computer science) , allergy , immunoglobulin e , sting , adverse effect , stimulation , gastroenterology , allergen , immunopathology , pharmacology , antibody , chemistry , microrna , biochemistry , microvesicles , computer science , engineering , gene , programming language , aerospace engineering
Background: Systemic side‐effects of venom immunotherapy (VIT) represent a considerable problem in the treatment of patients allergic to Hymenoptera venom. We examined the hypothesis whether basophil responsiveness might be connected with the adverse reactions to VIT. Methods: Basophil surface expression of activation marker CD63 induced by different concentrations of honeybee and wasp venom (0.1 and 1 μ g/ml) was measured by flow cytometry in 34 patients with history of systemic anaphylactic reactions to Hymenoptera sting just before rush honeybee or wasp VIT. Results: Eleven of 34 patients had systemic anaphylactic reaction (Mueller grades I–III) and one patient a large local reaction to VIT. In those 12 patients, median percentage of activated basophils after stimulation with VIT‐specific venom in concentration of 0.1 μ g/ml was 99% (range: 17–195) of value reached with stimulation with 1 μ g/ml. Side‐effects occurred in all patients with 0.1/1 ratios over 92% (eight of 12). In contrast, in 22 patients with no side‐effects, the median 0.1/1 ratio was 25% (range: 2–92). These concentration‐dependent activation ratios were significantly different between the groups with and without side reactions ( P < 0.0001). We also show significant positive correlation of the occurrence/clinical grade of the side‐effects with individual ratios of CD63 basophil response ( r = 0.73, P < 0.0001). Conclusion: The results suggest that increased basophil sensitivity to allergen‐specific in vitro stimulation is significantly associated with major side‐effects of VIT.