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Relationships between specific serum IgE, cytokines and polymorphisms in the IL‐4, IL‐4R α in patients with penicillins allergy
Author(s) -
Qiao H.L.,
Yang J.,
Zhang Y.W.
Publication year - 2005
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2005.00816.x
Subject(s) - immunoglobulin e , immunology , allergy , atopy , interleukin 4 , medicine , radioallergosorbent test , allele , interleukin , antibody , cytokine , biology , gene , genetics
Background: Excessive production of interleukin (IL)‐4, IL‐13 and interferon (IFN)‐ γ is thought to be important in the development of allergic disease and atopy. Several investigators have linked the IL‐4 and IL‐4R genes to allergic disease and atopy. The aim of this study is to further explore the mechanism of penicillins allergy and evaluate the possible role of the IL‐4 C‐589T and IL‐4R α Q576R polymorphisms in modulating the allergic responses to penicillins. Methods: Radioallergosorbent test (RAST) was used to detect eight kinds of specific immunoglobulin E (IgE) to penicillins in serum. Serum levels of IL‐4, IL‐13 and IFN‐ γ were measured by using enzyme‐linked immunosorbent assay (ELISA). The IL‐4 C‐589T and IL‐4R α Q576R polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP). Results: Compared with control subjects, there were significantly higher levels of IL‐4, IL‐13 and IFN‐ γ in allergic patients with positive specific IgE ( P < 0.01), and the lower levels of IL‐4 and IFN‐ γ were observed in allergic patients with negative specific IgE ( P < 0.05). We found a growing trend of IL‐4 and IL‐13 levels with the kind increasing of positive specific IgE, and even there were significant correlations between the three kinds of cytokines and many kinds of specific IgE ( P < 0.05). The IL‐4R α *Q576 allele was significantly increased in patients with penicillins allergy compared with control subjects ( P < 0.01). Furthermore, the allele was strongly associated with increased serum‐specific benzylpenicilloyl (BPO)‐, phenoxomethylpenicillanyl (PVA)‐ or ampicillanyl (APA)‐IgE levels in patients with positive specific IgE ( P < 0.05). Conclusions: These data suggest that IL‐4, IL‐13 and IFN‐ γ play an important roles in penicillins allergy. The IL‐4R α Q576R polymorphism may involve in the development of penicillins allergy, and through modulating specific serum IgE levels.