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Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics
Author(s) -
Profita M.,
Gagliardo R.,
Di Giorgi R.,
Pompeo F.,
Gjomarkaj M.,
Nicolini G.,
Bousquet J.,
Vignola A. M.
Publication year - 2005
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2005.00702.x
Subject(s) - salbutamol , formoterol , pharmacology , chemistry , bronchodilator , medicine , glucocorticoid receptor , endocrinology , receptor , corticosteroid , asthma , budesonide
Background: Several in vitro studies demonstrate that corticosteroids and long‐acting β 2 agonists may have a complementary and synergistic mode of action on the inflammatory processes in asthma. Methods: Sputum was induced in 20 mild to moderate asthmatic patients and the induced sputum cells (ISC) were cultured with beclomethasone dipropionate (BDP) 10 −7 M, salbutamol 10 −8 M and formoterol 10 −8 M either alone or in combination, BDP plus salbutamol and BDP plus formoterol, for 24 h. We measured the levels of growth macrophages‐colony stimulating factor (GM‐CSF), released on activation normal T cells expressed and activated (RANTES) and interleukin‐8 (IL‐8), in the supernatant of stimulated cells by ELISA. Furthermore, we assessed nuclear translocation of glucocorticoid receptor (GR) and the expression of β 2 receptor in ISC by immunofluorescence and RT‐PCR, respectively. Results: The release of GM‐CSF, RANTES and IL‐8 in ISC was significantly reduced by BDP plus salbutamol or formoterol as compared with either drug alone ( P < 0.0001). β 2 receptor expression was increased after 30 min of incubation with BDP and continued to increase over a time period of 4 h ( P < 0.0001). Furthermore after 30 min of incubation, nuclear translocation of GR was greater with BDP plus salbutamol or formoterol than with any of the drugs alone ( P < 0.0001). Conclusion: The present ex vivo study demonstrates a complementary mode of action between BDP and salbutamol or formoterol leading to an enhanced anti‐inflammatory activity.