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Comparative immunophenotyping of monocytes from symptomatic and asymptomatic atopic individuals
Author(s) -
Von Bubnoff D.,
Scheler M.,
Hinz T.,
Matz H.,
Koch S.,
Bieber T.
Publication year - 2004
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.2004.00546.x
Subject(s) - immunophenotyping , asymptomatic , medicine , immunology , atopy , allergy , dermatology , pathology , flow cytometry
Background: Allergy has at least two components – a genetic predisposition referred to as atopy and the progress from an atopic state to clinically apparent disease. Peripheral blood monocytes are circulating myeloid precursors of antigen‐presenting cells. The expression of cell surface proteins on monocytes may therefore witness the disease status and affect the development of allergic disease. Methods: Monocytes were isolated from atopic individuals with seasonal allergic rhinitis ( n = 10), from atopic individuals sensitized to aeroallergens but without any signs of acute disease ( n = 11), and from healthy nonatopic donors ( n = 21). Detailed comparative phenotypic analysis of CD14 + and Fc ɛ RI + CD14 + monocytes was performed by flow cytometry. Results: CD14 + monocytes from symptomatic atopic donors showed a significant increase in the cell surface intensity of the integrin adhesion molecule CD11c over monocytes from asymptomatic atopic and nonatopic donors. Asymptomatic atopic individuals showed significantly enhanced expression of Fc ɛ RI on the CD14 high CD16 dim monocyte subset compared with this subset from symptomatic atopic and nonatopic donors. Conclusion: The increase in monocyte surface intensity of the adhesion molecule CD11c may be involved in the manifestation of allergic disease. Fc ɛ RI on CD14 high CD16 dim monocytes of asymptomatic atopic donors may be of functional importance for the maintenance of clinical unresponsiveness toward allergens.