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Priming and inducing effects of interleukin‐3 on histamine release from cord‐blood basophils
Author(s) -
Miadonna A.,
Salmaso C.,
Di Marco M. P.,
Pugni L,
Milazzo N.,
Tedeschi A.,
Marini A.
Publication year - 1997
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1997.tb02419.x
Subject(s) - histamine , immunoglobulin e , cord blood , medicine , immunology , basophil , allergy , sensitization , priming (agriculture) , endocrinology , antibody , biology , germination , botany
The effects of inlerleukin‐3 (IL‐3) on histamine release from cord and adult blood basophils were evaluated. Leukocyte suspensions, obtained from adult patients with respiratory allergy ( n = 15), normal adult subjects ( n = 15), and neonates with ( n = 15) and without ( n = 19) atopic disposition, were stimulated with anti‐IgE, fMLP, and IL‐3. IgE‐mediated histamine release was significantly higher in adult patients, either allergic or normal, than in neonates with or without atopic disposition. A trend toward higher fMLP‐induced histamine release was found in allergic adult subjects. IL‐3 had a weak direct histamine‐releasing activity in allergic adult subjects and in neonates, but not in normal adult donors. A significant enhancing effect of IL‐3 on histamine release induced by anti‐IgE was observed in neonates with and without atopic disposition and in normal adult subjects, but not in atopic adult patients. IL‐3 exerted a priming effect also when basophils were stimulated with fMLP, without any significant difference between neonates and adult subjects. Passive sensitization with IgE‐rich serum resulted in a significant increase in anti‐IgE‐induced, but not in IL‐3–induced, histamine release from cord‐blood basophils. In conclusion, IL‐3 primes cord‐blood as well as adult blood basophils for a consecutive anti‐IgE‐ or fMLP‐induced histamine release, and its activity is not limited by the low density of membrane IgE in cord‐blood basophils.